http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15079922
Chudoku Kenkyu. 2004 Jan;17(1):51-4.
[A case of acute poisoning caused by the
inhalation of a nonselective herbicide REBIN GT SC (butafenacil
and glyphosate isopropylamine)]
[Article in Japanese]
Ishiguro M, Mikasa S, Otani M.
REBIN GT SC (glyphosate isopropylamine and butafenacil, hereinafter
referred to as "REBIN") is a nonselective herbicide
which was developed by Syngenta Japan K. K. and was registered
in July 2001 as a herbicide. We report the first case of acute
poisoning by REBIN. In this case (Age 67, male), high fever and
general fatigue developed immediately after REBIN inhalation.
Furthermore, urine sugar, urine protein, high LDH and high GPT
were observed. But the patient showed a tendency of recovery after
the lapse of 48 hours by the intravenous fluid replacement, hydrocortisone
sodium succinate (Solu-Cortef), glycyrrhizin (Stronger Neo-Minophagen
C) and glutathione (Tathion). He recovered satisfactorily. It
is necessary to respect the instructions for the use of REBIN.
In addition, in order to prevent inhalation of REBIN, the proper
use of instruments and spray at about 30 cm under knee are essential.
Publication Types:
• Case Reports
PMID: 15079922 [PubMed - indexed for MEDLINE]
http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12972658&dopt=Abstract
Plant Physiol. 2003
Sep
11 [Epub ahead of print].
Development
of Protoporphyrinogen Oxidase as an Efficient Selection Marker
for Agrobacterium tumefaciens-Mediated Transformation of Maize.
Li
X, Volrath SL, Nicholl DB, Chilcott CE, Johnson MA, Ward ER, Law
MD.
Syngenta
Biotechnology, Inc.,
P.O. Box 12257, 3054 Cornwallis Road, Research Triangle Park,
North Carolina 27709-2257.
In this article, we
report the isolation of plant protoporphyrinogen oxidase (PPO)
genes and the isolation of herbicide-tolerant mutants. Subsequently,
an Arabidopsis double mutant (Y426M + S305L) was used to develop
a selectable marker system for Agrobacterium tumefaciens-mediated
transformation of maize (Zea mays) and to obtain multiple events
tolerant to the PPO family of herbicides. Maize transformants
were produced via butafenacil selection using a flexible light
regime to increase selection pressure. Butafenacil selection per
se did not change transgene copy number distribution relative
to other selectable marker systems, but the most tolerant events
identified in the greenhouse were more likely to contain multiple
copies of the introduced mutant PPO gene. To
date, more than 2,500 independent transgenic maize events have
been produced using butafenacil selection. The high frequency
of A. tumefaciens-mediated transformation via PPO selection enabled
us to obtain single-copy transgenic maize lines tolerant to field
levels of butafenacil.
PMID: 12972658
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11851913&dopt=Abstract
Plant J. 2001
Dec;28(6):671-7.
Gene targeting
in Arabidopsis.
Hanin
M, Volrath S, Bogucki A, Briker M, Ward E, Paszkowski J.
Friedrich Miescher
Institute, Maulbeerstrasse 66, CH-4058 Basel, Switzerland. moez.hanin@fmi.ch
Precise modification
by gene targeting (GT) provides an important tool for studies
of gene function in vivo. Although routine with many organisms,
only isolated examples of GT events have been reported for flowering
plants. These were at low frequencies precluding reliable estimation
of targeting efficiency and evaluation of GT mechanisms. Here
we present an unambiguous and straightforward system for detection
of GT events in Arabidopsis using an endogenous nuclear gene encoding
protoporphyrinogen oxidase (PPO), involved in chlorophyll and
heme syntheses. Inhibition of PPO by the
herbicide Butafenacil results in rapid plant death. However,
the combination of two particular mutations renders PPO highly
resistant to Butafenacil. We exploited this feature for selection
of GT events by introducing the mutations into the PPO gene by
homologous recombination. We have estimated the basal GT frequency
to be 2.4 x 10(-3). Approximately one-third of events were true
GT (TGT) leading to the anticipated modification of the chromosomal
PPO copy. The remaining events could be classified as ectopic
GT (EGT) arising by modification of vector DNA by the chromosomal
template and its random integration into the Arabidopsis genome.
Thus the TGT frequency in our experimental setup is 0.72 x 10(-3).
In view of the high efficiency of Arabidopsis transformation,
GT experiments of a reasonable size followed by a PCR screen for
GT events should also allow for modification of non-selectable
targets. Moreover, the system presented here should contribute
significantly to future improvement of GT technology in plants.
PMID: 11851913
[PubMed - indexed for MEDLINE]